October 7, 2020

(Updated 10/26/20)

(Advisory: See Update to Article, The Zika Virus Scare: An Anatomy Of How Diseases Are Manufactured For Political Objectives)

The China Syndrome 4: I've Seen The [Green] Light! 

No software-based test can identify a sequence of DNA nucleotides as belonging to any particular pathogen, as inadvertently admitted by software programs (FASTA and BLAST being two such widely used software programs) that search for similarities when running a Query and Database Sequence, searching for a match between a database of billions of nucleotide sequences with that of the target nucleotide sequence being amplified. Similarity* informs us the target nucleotide sequence can't be identified ('seen') by the software, otherwise there would be a precise match with the database nucleotide sequences.

For Research Use Only. Not For Use In Diagnostic Procedures. 

The qPCR non-test accomplishes what it is manufactured for: Amplifying a nucleotide sequence and using green fluorescent dye to determine quantity, not identity. When amplification is, let’s say, between 1x to 20x the software gives a negative result because it can’t detect the green dye. When amplification is increased to 35x the qPCR non-test will give a positive result approximately 5% of the time because it can detect the green dye. At 50x amplification the qPCR non-test will give a 75% positivity rate. When amplification is increased to 60x, the qPCR non-test gives 100% positivity.

SYBR® Green I is a commonly used fluorescent dye that binds double-stranded DNA molecules by intercalating between the DNA bases. It is used in quantitative PCR because the fluorescence can be measured at the end of each amplification cycle to determine, relatively or absolutely, how much DNA has been amplified.

Real-Time PCR Fluorescence Detection [Emphasis: Mine] Systems

Without the green fluorescent dye, the qPCR non-test is incapable of even 'seeing'/detecting the billions of nucleotide sequences created, let alone 'see'/detect a particular target nucleotide sequence to determine if similarities exist between the target nucleotide sequence and the databank nucleotide sequences. If the green fluorescent dye is removed from the qPCR non-test, the result of the non-test will show 0% positivity 100% of the time! 

Is That You, Disease?

5' nuclease assay specificity Assay specificity is the degree that the assay includes signal from the target and excludes signal from non-target in the results. Specificity is arguably the most important aspect of any assay. The greatest threat to assay specificity for 5' nuclease assays is homologs. Homologs are genes similar in sequence to that of the target, but they are not the intended target of the assay. Homologs are extremely common within species and across related species.

...

5' nuclease assays offer two tools for specificity: primers and probes. For maximal impact on specificity by primers, a mismatch between the target and homolog must be positioned at the 3' -most base of the primer. A mismatch further away from the 3’ end may have little to no impact on specificity. In contrast, mismatches across most of the length of a MGB probe, which is shorter than a TaqMan® probe, can have a strong impact on specificity—TaqMan® MGB probes are stronger tools for specificity than primers. 

For example, a one- or two-base random mismatch in the primer binding site will very likely allow the DNA polymerase to extend the primer bound to the homolog with high efficiency. A one or two base extension by DNA polymerase will stabilize the primer bound to the homolog, so it is just as stably bound as primer bound to the intended, fully complementary target. At that point, there is nothing to prevent the DNA polymerase from continuing synthesis to produce a copy of the homolog.

Of course, the above quote accepts as true that the target is indeed the target, but in the case of the pathogen referred to as COVID-19, it doesn't exist,** meaning the target is mismatched from the beginning when the swab enters the nasal cavity! 

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* Imagine criminal forensic science requiring only similarities for fingerprint identification! Yeah, just as with the false positive laden qPCR test, 100% of the nation would be guilty of a crime every time a fingerprint check was run! Or how about forensic DNA matching requiring only similarities! Imagine running a forensic ballistic test and only looking for similarities in bullet striations! Now one can fully appreciate the fraud behind the qPCR non-test.

** In July 2020, the CDC admitted there is no isolation of COVID-19, therefore there can't exist a pathogen referenced as COVID-19:

Since no quantified virus isolates of the 2019-nCoV are currently available...